Antimicrobial Peptides from Skin Secretions of Hypsiboas pulchellus (Anura: Hylidae)

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• 作者：Alvaro Siano ; Mar铆a Ver贸nica H煤mpola ; Eliandre de Oliveira ; Fernando Albericio ; Arturo C. Simonetta ; Rafael Lajmanovich ; Georgina G. Tonarelli
• 刊名：Journal of Natural Products
• 出版年：2014
• 出版时间：April 25, 2014
• 年：2014
• 卷：77
• 期：4
• 页码：831-841
• 全文大小：454K
• 年卷期：v.77,no.4(April 25, 2014)
• ISSN：1520-6025

文摘

The skin of many amphibians produces a large repertoire of antimicrobial peptides that are crucial in the first line of defense against microbial invasion. Despite the immense richness of wild amphibians in Argentina, knowledge about peptides with antimicrobial properties is limited to a few species. Here we used LC-MS-MS to analyze samples of Hypsiboas pulchellus skin with the aim to identify antimicrobial peptides in the mass range of 1000 to 2000 Da. Twenty-three novel sequences were identified by MS, three of which were selected for chemical synthesis and further studies. The three synthetic peptides, named P1-Hp-1971, P2-Hp-1935, and P3-Hp-1891, inhibited the growth of two ATCC strains: Escherichia coli (MIC: 16, 33, and 17 渭M, respectively) and Staphylococcus aureus (MIC: 8, 66, and 17 渭M, respectively). P1-Hp-1971 and P3-Hp-1891 were the most active peptides. P1-Hp-1971, which showed the highest therapeutic indices (40 for E. coli and 80 for S. aureus), is a proline-glycine-rich peptide with a highly unordered structure, while P3-Hp-1891 adopts an amphipathic 伪-helical structure in the presence of 2,2,2-trifluoroethanol and anionic liposomes. This is the first peptidomic study of Hypsiboas pulchellus skin secretions to allow the identification of antimicrobial peptides.