Quantitative Side-Chain Modifications of Methionine-Containing Elastin-Like Polypeptides as a Versatile Tool to Tune Their Properties
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- 作者：Jessica R. Kramer ; Rosine Petitdemange ; Laure Bataille ; Katell Bathany ; Anne-Laure Wirotius ; Bertrand Garbay ; Timothy J. Deming ; Elisabeth Garanger ; Sebastien Lecommandoux
- 刊名：ACS Macro Letters
- 出版年：2015
- 出版时间：November 17, 2015
- 年：2015
- 卷：4
- 期：11
- 页码：1283-1286
- 全文大小：304K
- ISSN：2161-1653
文摘
Tuning the lower critical solution temperature (LCST) of temperature-responsive recombinant elastin-like polypeptides has usually been achieved by designing different protein sequences, in terms of amino acid composition and length, implying tedious molecular cloning steps. In the present work, we have explored the chemoselective alkylation of methionine as an easy means to modify elastin repeat side chains and easily modulate the LCST of the polypeptides. Such a versatile synthetic method shall practically be exploited to modulate any properties of recombinant polymers.