In Vitro Selection for Small-Molecule-Triggered Strand Displacement and Riboswitch Activity
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- 作者：Laura Martini ; Adam J. Meyer ; Jared W. Ellefson ; John N. Milligan ; Michele Forlin ; Andrew D. Ellington ; Sheref S. Mansy
- 刊名：ACS Synthetic Biology
- 出版年：2015
- 出版时间：October 16, 2015
- 年：2015
- 卷：4
- 期：10
- 页码：1144-1150
- 全文大小：287K
- ISSN：2161-5063
文摘
An in vitro selection method for ligand-responsive RNA sensors was developed that exploited strand displacement reactions. The RNA library was based on the thiamine pyrophosphate (TPP) riboswitch, and RNA sequences capable of hybridizing to a target duplex DNA in a TPP regulated manner were identified. After three rounds of selection, RNA molecules that mediated a strand exchange reaction upon TPP binding were enriched. The enriched sequences also showed riboswitch activity. Our results demonstrated that small-molecule-responsive nucleic acid sensors can be selected to control the activity of target nucleic acid circuitry.