Analysis of Microbial Components Using LC-IR

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• 作者：Scott W. Huffman ; Kara Lukasiewicz ; Susan Geldart ; Susan Elliott ; Jay F. Sperry ; and Chris W. Brown
• 刊名：Analytical Chemistry
• 出版年：2003
• 出版时间：September 1, 2003
• 年：2003
• 卷：75
• 期：17
• 页码：4606 - 4611
• 全文大小：99K
• 年卷期：v.75,no.17(September 1, 2003)
• ISSN：1520-6882

文摘

Characterization of bacteria is currently an importantresearch area in the medical, military, food, and agricultural sciences. In recent years, FT-IR has found anapplication as a microbiological detection method and asa general research tool. When coupled with a liquidchromatographic system, a new facet of research hasevolved. By utilizing the separation ability of typical liquidchromatography systems, matrix elimination is possible,therefore allowing for clean spectra of cellular components. Information about the compositional makeup ofvarious bacteria enhances the overall understanding ofbiology at the cellular level, provides a quantification ofthe chemistry of cellular processes, and can be used as ageneral identification tool. Both whole cells and lysedEscherichia coli cells were investigated in the presentstudy. The cellular components consisting of proteins,glycoproteins, phospholipids, fatty amides and acids, andgenomic materials were separated, isolated, and identifiedby FT-IR.