Proteomic Characterization of Midproliferative and Midsecretory Human Endometrium
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- 作者：Jenny I-C. Chen* ; Natalie J. Hannan ; Yunxian Mak ; Peter K. Nicholls ; Jin Zhang ; Adam Rainczuk ; Peter G. Stanton ; David M. Robertson ; Lois A. Salamonsen ; Andrew N. Stephens
- 刊名：Journal of Proteome Research
- 出版年：2009
- 出版时间：April 3, 2009
- 年：2009
- 卷：8
- 期：4
- 页码：2032-2044
- 全文大小：632K
- 年卷期：v.8,no.4(April 3, 2009)
- ISSN：1535-3907
文摘
This study aimed to identify proteins differentially expressed in the human endometrium between the proliferative and secretory phases of normal menstrual cycles by 2D differential in-gel electrophoresis (DIGE). A total of 196 out of 1017 spots were differentially expressed (p < 0.05). Mass spectrometry identified 76 proteins representing 41 different gene products. Immunohistochemistry confirmed the observed changes in 3 representative proteins (Rho-GDIα, CLIC1, PGRMC1). Biological pathway analysis identified the Jnk and EGF signaling pathways as key regulators of protein expression in the midsecretory phase of endometrial proteome.