Parallel On-Chip Analysis of Single Vesicle Neurotransmitter Release

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• 作者：Alexey Yakushenko ; Enno K盲telh枚n ; Bernhard Wolfrum
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：June 4, 2013
• 年：2013
• 卷：85
• 期：11
• 页码：5483-5490
• 全文大小：404K
• 年卷期：v.85,no.11(June 4, 2013)
• ISSN：1520-6882

文摘

Real-time investigations of neurotransmitter release provide a direct insight on the mechanisms involved in synaptic communication. Carbon fiber microelectrodes are state-of-the-art tools for electrochemical measurements of single vesicle neurotransmitter release. Yet, they lack high-throughput capabilities that are required for collecting robust statistically significant data across multiple samples. Here, we present a chip-based recording system enabling parallel in vitro measurements of individual neurotransmitter release events from cells, cultured directly on planar multielectrode arrays. The applicability of this cell-based platform to pharmacological screening is demonstrated by resolving minute concentration-dependent effects of the dopamine reuptake inhibitor nomifensine on recorded single-vesicle release events from PC12 cells. The experimental results, showing an increased half-time of the recorded events, are complemented by an analytical model for the verification of drug action.