Fluorescent Detection of Lead in Environmental Water and Urine Samples Using Enzyme Mimics of Catechin-Synthesized Au Nanoparticles

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• 作者：Yan-Shiuan Wu ; Fan-Feng Huang ; Yang-Wei Lin
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2013
• 出版时间：February 27, 2013
• 年：2013
• 卷：5
• 期：4
• 页码：1503-1509
• 全文大小：355K
• 年卷期：v.5,no.4(February 27, 2013)
• ISSN：1944-8252

文摘

A facile, cost-effective, and sensitive fluorescent method for Pb²⁺ ion detection had been developed using catechin synthesized gold nanoparticles (C鈥揂u NPs). The Pb鈥揷atechin complexes and Pb鈥揂u alloys that formed on the C鈥揂u NPs surfaces allowed NPs to exhibit peroxidase-mimicking catalytic activity in the H₂O₂-mediated oxidation of Amplex UltraRed (AUR). In 5 mM Tris-acetate buffers at pH 7.0, the H₂O₂鈥揂UR鈥揅鈥揂u NP probe was highly selective (>100-fold) for Pb²⁺ ions in the presence of other tested metal ions (K⁺, Ag⁺, Na⁺, Cd²⁺, Ni²⁺, Ca²⁺, Hg²⁺, Sr²⁺, Co²⁺, Cu²⁺, Ba²⁺, Fe²⁺, Mg²⁺, Cr³⁺, and Fe³⁺ ions). The fluorescence intensity (excitation/emission maxima 540/588 nm) of the AUR product was proportional to the concentration of Pb²⁺ ions in the range of 10 nM鈥?.0 渭M with a linear correlation (R² = 0.99). The H₂O₂鈥揂UR鈥揅鈥揂u NP probe detected Pb²⁺ ions with a limit of detection (signal-to-noise ratio: 3) of 1.5 nM. The practicality of the H₂O₂鈥揂UR鈥揅鈥揂u NP probe was validated for the determination of Pb²⁺ ion concentration in environmental water and urine samples, demonstrating its advantages of simplicity, selectivity, and sensitivity.

Keywords:

Amplex UltraRed; catechin; lead(II); environmental water samples; urine sample