Quantitative Proteomic and Phosphoproteomic Approaches for Deciphering the Signaling Pathway for Tension Wood Formation in Poplar

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• 作者：M茅lanie Mauriat ; Jean-Charles Lepl茅 ; St茅phane Claverol ; J茅r么me Bartholom茅 ; Luc Negroni ; Nicolas Richet ; C茅line Lalanne ; Marc Bonneu ; Catherine Coutand ; Christophe Plomion
• 刊名：Journal of Proteome Research
• 出版年：2015
• 出版时间：August 7, 2015
• 年：2015
• 卷：14
• 期：8
• 页码：3188-3203
• 全文大小：501K
• ISSN：1535-3907

文摘

Trees adjust their growth following forced changes in orientation to re-establish a vertical position. In angiosperms, this adjustment involves the differential regulation of vascular cambial activity between the lower (opposite wood) and upper (tension wood) sides of the leaning stem. We investigated the molecular mechanisms leading to the formation of differential wood types through a quantitative proteomic and phosphoproteomic analysis on poplar subjected to a gravitropic stimulus. We identified and quantified 675 phosphopeptides, corresponding to 468 phosphoproteins, and 3鈥?63 nonphosphorylated peptides, corresponding to 1鈥?55 proteins, in the differentiating xylem of straight-growing trees (control) and trees subjected to a gravitational stimulus during 8 weeks. About 1% of the peptides were specific to a wood type (straight, opposite, or tension wood). Proteins quantified in more than one type of wood were more numerous: a mixed linear model showed 389 phosphopeptides and 556 proteins to differ in abundance between tension wood and opposite wood. Twenty-one percent of the phosphoproteins identified here were described in their phosphorylated form for the first time. Our analyses revealed remarkable developmental molecular plasticity, with wood type-specific phosphorylation events, and highlighted the involvement of different proteins in the biosynthesis of cell wall components during the formation of the three types of wood.