Direct Profiling and Imaging of Epicuticular Waxes on Arabidopsis thaliana by Laser Desorption/Ionization Mass Spectrometry Using Silver Colloid as a Matrix

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• 作者：Sangwon Cha ; Zhihong Song ; Basil J. Nikolau ; Edward S. Yeung
• 刊名：Analytical Chemistry
• 出版年：2009
• 出版时间：April 15, 2009
• 年：2009
• 卷：81
• 期：8
• 页码：2991-3000
• 全文大小：372K
• 年卷期：v.81,no.8(April 15, 2009)
• ISSN：1520-6882

文摘

Colloidal silver laser desorption/ionization (LDI) mass spectrometry (MS) was employed to directly profile and image epicuticular wax metabolites on a variety of different surfaces of Arabidopsis thaliana leaves and flowers. Major cuticular wax compounds, such as very long-chain fatty acids, alcohols, alkanes, and ketones, were successfully detected as silver adduct ions. The surface metabolites of different flower organs (carpels, petals, and sepals) were profiled for the first time at a spatial resolution of 100 μm. In addition, mass spectral profiles and images were collected from wild type and a mutant strain, which carried alleles that affect the surface constituents of this organism. One of these mutant alleles (cer2-2) is in a gene whose biochemical functionality is still unclear, although its effect on normal epicuticular wax deposition was the characteristic that led to its original identification. Variations of wax products between different spatial locations for wild type and for a mutant strain were investigated by normalizing the ion intensities to a reference peak ([¹⁰⁷Ag + ¹⁰⁹Ag]⁺). The spatially resolved surface metabolite profiling data of this mutant has provided new insights into the complexity of epicuticular wax deposition at the cellular-resolution scale. This MS-based metabolite imaging technology has the potential to provide valuable data for dissecting metabolism in multicellular organism at the level of single cells.