Comparison of Polyacetylene Content in Organically and Conventionally Grown Carrots Using a Fast Ultrasonic Liquid Extraction Method
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- 作者：Malene Sø ; ltoft ; Morten Rosbjø ; rn Eriksen ; Anne Wibe Bræ ; ndholt Trger ; John Nielsen ; Kristian Holst Laursen ; Sø ; ren Husted ; Ulrich Halekoh ; Pia Knuthsen
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2010
- 出版时间：July 14, 2010
- 年：2010
- 卷：58
- 期：13
- 页码：7673-7679
- 全文大小：754K
- 年卷期：v.58,no.13(July 14, 2010)
- ISSN：1520-5118
文摘
A rapid and sensitive analytical method for quantification of polyacetylenes in carrot roots was developed. The traditional extraction method (stirring) was compared to a new ultrasonic liquid processor (ULP)-based methodology using high-performance liquid chromatography−ultraviolet (HPLC−UV) and mass spectrometry (MS) for identification and quantification of three polyacetylenes. ULP was superior because a significant reduction in extraction time and improved extraction efficiencies were obtained. After optimization, the ULP method showed good selectivity, precision [relative standard deviations (RSDs) of 2.3−3.6%], and recovery (93% of falcarindiol) of the polyacetylenes. The applicability of the method was documented by comparative analyses of carrots grown organically or conventionally in a 2 year field trial study. The average concentrations of falcarindiol, falcarindiol-3-acetate, and falcarinol in year 1 were 222, 30, and 94 μg of falcarindiol equiv/g of dry weight, respectively, and 3−15% lower in year 2. The concentrations were not significantly influenced by the growth system, but a significant year−year variation was observed for falcarindiol-3-acetate.