A study was conducted to inv
estigate the amino acid (AA) composition and the susceptibi
lity to in vitro proteo
lysis (pepsin, 120 min and pancreatin, 240 min) of a co
llection of purified phaseo
lins (
n = 43) in unheated or heat-treated form. The AA composition of phaseo
lin varied
litt
le across bean varieties. At 360 min of in vitro proteo
lysis, the degree of hydro
lysis varied from 11 to 27% for unheated and from 57 to 96% for heated phaseo
lins (
P &
lt; 0.001). Heat treatment marked
ly increased the susceptibi
lity of phaseo
lin to proteo
lysis (
P &
lt; 0.001). The AA scores (AAS) and the protein dig
estibi
lity corrected for AAS indicated S-containing AA as the
limiting AA (39 ± 3 and 30 ± 5%, respective
ly). In conc
lusion, susceptibi
lity to proteo
lysis of heat-treated phaseo
lin rather than its AA composition affects the nutritiona
l va
lue of phaseo
lin
estimated in vitro. Therefore, it shou
ld be the criterion of choice in breeding programs aimed at improving the nutritiona
l va
lue of common beans for humans.
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