Fluorescence Lifetime Standards for Time and Frequency Domain Fluorescence Spectroscopy
详细信息 查看全文
- 作者：Noë ; l Boens ; Wenwu Qin ; Nikola Basari
//pubs.acs.org/images/entities/cacute.gif" border="0"> ; Johan Hofkens ; Marcel Ameloot ; Jacques Pouget ; Jean-Pierre Lefè ; vre ; Bernard Valeu
- 刊名：Analytical Chemistry
- 出版年：2007
- 出版时间：March 1, 2007
- 年：2007
- 卷：79
- 期：5
- 页码：2137 - 2149
- 全文大小：178K
- 年卷期：v.79,no.5(March 1, 2007)
- ISSN：1520-6882
文摘
A series of fluorophores with single-exponential fluorescence decays in liquid solution at 20 
C were measuredindependently by nine laboratories using single-photontiming and multifrequency phase and modulation fluorometry instruments with lasers as excitation source. Thedyes that can serve as fluorescence lifetime standards fortime-domain and frequency-domain measurements are allcommercially available, are photostable under the conditions of the measurements, and are soluble in solvents ofspectroscopic quality (methanol, cyclohexane, water).These lifetime standards are anthracene, 9-cyanoanthracene, 9,10-diphenylanthracene, N-methylcarbazole,coumarin 153, erythrosin B, N-acetyl-L-tryptophanamide,1,4-bis(5-phenyloxazol-2-yl)benzene, 2,5-diphenyloxazole,rhodamine B, rubrene, N-(3-sulfopropyl)acridinium, and1,4-diphenylbenzene. At 20 C, the fluorescence lifetimesvary from 89 ps to 31.2 ns, depending on fluorescent dyeand solvent, which is a useful range for modern pico- andnanosecond time-domain or mega- to gigahertz frequency-domain instrumentation. The decay times are independent of the excitation and emission wavelengths. Dependent on the structure of the dye and the solvent, theexcitation wavelengths used range from 284 to 575 nm,the emission from 330 to 630 nm. These lifetime standards may be used to either calibrate or test the resolutionof time- and frequency-domain instrumentation or asreference compounds to eliminate the color effect inphotomultiplier tubes. Statistical analyses by means oftwo-sample charts indicate that there is no laboratory biasin the lifetime determinations. Moreover, statistical testsshow that there is an excellent correlation between thelifetimes estimated by the time-domain and frequency-domain fluorometries. Comprehensive tables compilingthe results for 20 (fluorescence lifetime standard/solvent)combinations are given.
C were measuredindependently by nine laboratories using single-photontiming and multifrequency phase and modulation fluorometry instruments with lasers as excitation source. Thedyes that can serve as fluorescence lifetime standards fortime-domain and frequency-domain measurements are allcommercially available, are photostable under the conditions of the measurements, and are soluble in solvents ofspectroscopic quality (methanol, cyclohexane, water).These lifetime standards are anthracene, 9-cyanoanthracene, 9,10-diphenylanthracene, N-methylcarbazole,coumarin 153, erythrosin B, N-acetyl-L-tryptophanamide,1,4-bis(5-phenyloxazol-2-yl)benzene, 2,5-diphenyloxazole,rhodamine B, rubrene, N-(3-sulfopropyl)acridinium, and1,4-diphenylbenzene. At 20 C, the fluorescence lifetimesvary from 89 ps to 31.2 ns, depending on fluorescent dyeand solvent, which is a useful range for modern pico- andnanosecond time-domain or mega- to gigahertz frequency-domain instrumentation. The decay times are independent of the excitation and emission wavelengths. Dependent on the structure of the dye and the solvent, theexcitation wavelengths used range from 284 to 575 nm,the emission from 330 to 630 nm. These lifetime standards may be used to either calibrate or test the resolutionof time- and frequency-domain instrumentation or asreference compounds to eliminate the color effect inphotomultiplier tubes. Statistical analyses by means oftwo-sample charts indicate that there is no laboratory biasin the lifetime determinations. Moreover, statistical testsshow that there is an excellent correlation between thelifetimes estimated by the time-domain and frequency-domain fluorometries. Comprehensive tables compilingthe results for 20 (fluorescence lifetime standard/solvent)combinations are given.