In Situ Visualization of Paclitaxel Distribution and Release by Coherent Anti-Stokes Raman Scattering Microscopy
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- 作者：Eunah Kang ; Haifeng Wang ; Il Keun Kwon ; Joshua Robinson ; Kinam Park ; Ji-Xin Cheng
- 刊名：Analytical Chemistry
- 出版年：2006
- 出版时间：December 1, 2006
- 年：2006
- 卷：78
- 期：23
- 页码：8036 - 8043
- 全文大小：1339K
- 年卷期：v.78,no.23(December 1, 2006)
- ISSN：1520-6882
文摘
Visualization of three-dimensional distribution of drugmolecules and subsequent changes during the releaseprocess is critical for understanding drug delivery mechanisms as well as designing tailor-made release profiles.This study utilized coherent anti-Stokes Raman scattering(CARS) imaging to examine paclitaxel distribution invarious polymer films with lateral resolution of 0.3 
mand depth resolution of 0.9 m. Raman bands in the CHstretch vibration and fingerprint regions were used todistinguish paclitaxel from the polymers. The detectionsensitivity was measured to be 29 mM by imaging paclitaxel molecules dissolved in N,N-dimethylformamidesolution. Release of paclitaxel from a polymer matrix wasmonitored at an acquisition speed of 1 frame/s. Ourresults show that CARS microscopy can be used effectivelyfor in situ imaging of native drug molecules in a deliverysystem.
mand depth resolution of 0.9 m. Raman bands in the CHstretch vibration and fingerprint regions were used todistinguish paclitaxel from the polymers. The detectionsensitivity was measured to be 29 mM by imaging paclitaxel molecules dissolved in N,N-dimethylformamidesolution. Release of paclitaxel from a polymer matrix wasmonitored at an acquisition speed of 1 frame/s. Ourresults show that CARS microscopy can be used effectivelyfor in situ imaging of native drug molecules in a deliverysystem.