Diverse Antimicrobial Killing by Enterococcus faecium E 50-52 Bacteriocin

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• 作者：Edward A. Svetoch ; Boris V. Eruslanov ; Vladimir V. Perelygin ; Evgeni V. Mitsevich ; Irina P. Mitsevich ; Valery N. Borzenkov ; Vladimir P. Levchuk ; Olga E. Svetoch ; Yuri N. Kovalev ; Yuri G. Stepanshin ; G
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2008
• 出版时间：March 26, 2008
• 年：2008
• 卷：56
• 期：6
• 页码：1942 - 1948
• 全文大小：1014K
• 年卷期：v.56,no.6(March 26, 2008)
• ISSN：1520-5118

文摘

An effective bacteriocin was identified and characterized. Lactic acid bacteria were screened against Campylobacter jejuni. One bacteriocin producer, Enterococcus faecium (NRRL B-30746), was studied. The isolate was grown, and the bacteriocin was purified to single-band homogeneity. Biochemical traits indicated that the peptide was a Class IIa bacteriocin, and it was named E 50-52. The bacteriocin had a molecular weight of 3339.7 and an isoelectric point of 8.0. The minimal inhibitory concentrations of E 50-52 against C. jejuni, Yersinia spp., Salmonella spp., Escherichia coli O157:H7, Shigella dysenteriae, Morganella morganii, Staphylococcus spp., and Listeria spp. ranged from 0.025 to 32 µg/mL. In therapeutic broiler trials, oral treatment with E 50-52 reduced both C. jejuni and Salmonella enteritidis by more than 100 000-fold in the ceca, and systemic S. enteritidis was reduced in the liver and spleen. The wide range of antibacterial activity of bacteriocin E 50-52 against pathogens provides a promising alternative to antibiotics.