Kinetic Model for a Threshold Filter in an Enzymatic System for Bioanalytical and Biocomputing Applications

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• 作者：Vladimir Privman ; Sergii Domanskyi ; Shay Mailloux ; Yaovi Holade ; Evgeny Katz
• 刊名：Journal of Physical Chemistry B
• 出版年：2014
• 出版时间：October 30, 2014
• 年：2014
• 卷：118
• 期：43
• 页码：12435-12443
• 全文大小：352K
• ISSN：1520-5207

文摘

A recently experimentally observed biochemical 鈥渢hreshold filtering鈥?mechanism by processes catalyzed by the enzyme malate dehydrogenase is explained in terms of a model that incorporates an unusual mechanism of inhibition of this enzyme that has a reversible mechanism of action. Experimental data for a system in which the output signal is produced by biocatalytic processes of the enzyme glucose dehydrogenase are analyzed to verify the model鈥檚 validity. We also establish that fast reversible conversion of the output product to another compound, without the additional inhibition, cannot on its own result in filtering.