Effects of a Chitin-Binding Vicilin from Enterolobium contortisiliquum Seeds on Bean Bruchid Pests (Callosobruchus maculatus and Zabrotes subfasciatus) and Phytopathogenic Fungi (
详细信息 查看全文
- 作者：Fabiano T. Moura ; Adeliana S. Oliveira ; Leonardo L. P. Macedo ; André ; L. B. R. Vianna ; Lucia B. S. Andrade ; A. S. Martins-Miranda ; Jose T. A. Oliveira ; Elizeu A. Santos ; Mauricio P. de Sales
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2007
- 出版时间：January 24, 2007
- 年：2007
- 卷：55
- 期：2
- 页码：260 - 266
- 全文大小：367K
- 年卷期：v.55,no.2(January 24, 2007)
- ISSN：1520-5118
文摘
Chitin-binding vicilin from Enterolobium contortisiliquum seeds was purified by ammonium sulfatefollowed by gel filtration on Sephacryl 300-SH and on Sephacryl 200-SH. The vicilin, called EcV, isa dimeric glycoprotein composed of 1.03% carbohydrates and a Mr of 151 kDa, consisting of twosubunits of Mr of 66.2 and 63.8 kDa. The EcV homogeneity was confirmed in a PAGE where it wasobserved to be a unique acid protein band with slow mobility in this native gel. E. contortisiliquumvicilin (EcV) was tested for anti-insect activity against C. maculatus and Zabrotes subfasciatus larvaeand for phytopathogenic fungi, F. solani and C. lindemuntianum. EcV was very effective against bothbruchids, producing 50% mortality for Z. subfasciatus at an LD50 of 0.43% and affected 50% of thelarvae mass with an ED50 of 0.65%. In artificial diets given to C. maculatus, 50% of the larvae masswas affected with an ED50 of 1.03%, and larva mortality was 50% at LD50 of 1.11%. EcV was notdigested by midgut homogenates of C. maculatus and Z. Subfasciatus until 12 h of incubation, andat 24 h EcV was more resistant to Z. subfasciatus larval proteases. The binding to chitin present inlarvae gut associated to low EcV digestibility could explain its lethal effects. EcV also exerted aninhibitory effect on the germination of F. solani at concentrations of 10 and 20 
g mL-1. The effectof EcV on fungi is possibly due to binding to chitin-containing structures of the fungal cell wall.
g mL-1. The effectof EcV on fungi is possibly due to binding to chitin-containing structures of the fungal cell wall.