PCR-RFLP Authentication of Meats from Red Deer (Cervus elaphus), Fallow Deer (Dama dama), Roe Deer (Capreolus capreolus), Cattle (Bos taurus), Sheep (Ovis aries
详细信息 查看全文
- 作者：Violeta Fajardo ; Isabel Gonzá ; lez ; Iné ; s Ló ; pez-Calleja ; Irene Martí ; n ; Pablo E. Herná ; ndez ; Teresa Garcí ; a ; and Rosario Martí ; n
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2006
- 出版时间：February 22, 2006
- 年：2006
- 卷：54
- 期：4
- 页码：1144 - 1150
- 全文大小：1654K
- 年卷期：v.54,no.4(February 22, 2006)
- ISSN：1520-5118
文摘
PCR-RFLP analysis has been applied to the identification of meats from red deer (Cervus elaphus),fallow deer (Dama dama), roe deer (Capreolus capreolus), cattle (Bos taurus), sheep (Ovis aries),and goat (Capra hircus). PCR amplification was carried out using a set of primers flanking a conservedregion of ~712 base pairs from the mitochondrial 12S rRNA gene. Restriction site analysis based onsequence data from this DNA fragment permitted the selection of MseI, MboII, BslI, and ApoIendonucleases for species identification. The restriction profiles obtained when amplicons weredigested with the chosen enzymes allowed the unequivocal identification of all domestic and gamemeat species analyzed in the present work.Keywords: Game meat; species identification; 12S rRNA gene; PCR-RFLP