In Vitro Pharmacological and Toxicological Effects of Norterpene Peroxides Isolated from the Red Sea Sponge Diacarnus erythraeanus on Normal and Cancer Cells

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• 作者：Florence Lefranc ; Genoveffa Nuzzo ; Nehal Aly Hamdy ; Issa Fakhr ; Laetitia Moreno Y Banuls ; Gwendoline Van Goietsenoven ; Guido Villani ; V茅ronique Mathieu ; Rob van Soest ; Robert Kiss ; Maria Letizia Ciavatta
• 刊名：Journal of Natural Products
• 出版年：2013
• 出版时间：September 27, 2013
• 年：2013
• 卷：76
• 期：9
• 页码：1541-1547
• 全文大小：363K
• 年卷期：v.76,no.9(September 27, 2013)
• ISSN：1520-6025

文摘

Eight cyclic peroxide norterpenoids, compounds 1鈥?b>8, have been isolated and characterized from the Red Sea sponge Diacarnus erythraeanus, including two new norsesterterpene derivatives (3, 4). Among these metabolites, (鈭?-muqubilin A (5) (nine cell lines analyzed) and the new compounds 3 and 4 (seven cell lines analyzed) displayed mean IC₅₀ growth inhibitory concentrations in vitro of <10 渭M, while the remaining compounds (1, 6鈥?b>8) were inactive in these cancer cell lines. Compound 5 displayed no selectivity between normal and cancer cells in terms of in vitro growth inhibition. Quantitative video microscopy analysis carried out on (鈭?-muqubilin A-treated cells validated the data obtained by means of the MTT colorimetric assay, while flow cytometry analysis revealed ROS production but no induction of apoptosis in cancer cells.