The Apoptotic Effect of Green Tea (-)-Epigallocatechin Gallate on 3T3-L1 Preadipocytes Depends on the Cdk2 Pathway
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- 作者：Bo-Tsung Wu ; Pei-Fang Hung ; Hui-Chian Chen ; Rong-Nan Huang ; Hsin-Huei Chang ; and Yung-Hsi Kao
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2005
- 出版时间：July 13, 2005
- 年：2005
- 卷：53
- 期：14
- 页码：5695 - 5701
- 全文大小：263K
- 年卷期：v.53,no.14(July 13, 2005)
- ISSN：1520-5118
文摘
This study was designed to investigate the effect of green tea catechins, especially (-)-epigallocatechingallate (EGCG), on the apoptosis of 3T3-L1 preadipocytes. Preadipocyte apoptosis as indicated byformation of DNA fragments was induced by EGCG in dose-dependent manners. While EGCG wasdemonstrated to decrease Cdk2 expression and activity and increase caspase-3 activity, overexpression of Cdk2 and treatment with the caspase-3 inhibitor respectively prevented preadipocytesfrom induction of DNA fragmentation and caspase-3 activity by doses of 100-400 
M of EGCG.This suggests the Cdk2- and caspase-3-dependent apoptotic effects of EGCG. Moreover, EGCGwas more effective than EC, ECG, and EGC in changing the apoptotic signals. Results of this studymay relate to the mechanism by which EGCG modulates body weight.Keywords: Green tea; epigallocatechin gallate; 3T3-L1 preadipocyte; cyclin-dependent kinase; caspase-3; apoptosis
M of EGCG.This suggests the Cdk2- and caspase-3-dependent apoptotic effects of EGCG. Moreover, EGCGwas more effective than EC, ECG, and EGC in changing the apoptotic signals. Results of this studymay relate to the mechanism by which EGCG modulates body weight.Keywords: Green tea; epigallocatechin gallate; 3T3-L1 preadipocyte; cyclin-dependent kinase; caspase-3; apoptosis