Supercritical Fluid Chromatography and High-Performance Liquid Chromatography/Tandem Mass Spectrometric Methods for the Determination of Cytarabine in Mouse Plasma
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- 作者：Yunsheng Hsieh ; Fangbiao Li ; Christine J. G. Duncan
- 刊名：Analytical Chemistry
- 出版年：2007
- 出版时间：May 15, 2007
- 年：2007
- 卷：79
- 期：10
- 页码：3856 - 3861
- 全文大小：131K
- 年卷期：v.79,no.10(May 15, 2007)
- ISSN：1520-6882
文摘
The separation of cytarabine (ara-C) from the endogenouscompounds in mouse plasma by packed-column supercritical fluid chromatography (pSFC) was achieved on baresilica stationary phase with an isocratic mobile phasecomposed of CO2/methanol solvent with addition ofammonium acetate. SFC is commonly assumed to be onlyapplicable to nonpolar and relatively low-polarity compounds. In this work, a broader range of compoundpolarities amenable to pSFC with appropriate mobile-phase modifiers and additives under normal-phase retention mechanism was demonstrated. The pSFC was integrated with an atmospheric pressure chemical ionizationsource and a tandem mass spectrometer (MS/MS) toenhance the sensitivity, selectivity, and speed of the assay.The influence of mobile-phase components on chromatographic performance and ionization efficiency of the testcompounds was investigated for improving the sensitivityand separation for the analyte and the internal standard.The pSFC-MS/MS approach requiring ~2.5 min/samplefor the determination of ara-C at nanograms per milliliterin mouse plasma was partially validated with respect tostability, linearity, and reproducibility. The mouse plasmalevels of ara-C obtained by the pSFC-MS/MS methodwere found to be consistent with those determined byvarious reversed-phase, high-performance liquid chromatography methods using a porous graphite carboncolumn, a mixed-mode column, or a C18 column inconjunction with an ion-pairing agent coupled to a tandemmass spectrometer.