Ratiometric Temperature Sensing with Semiconducting Polymer Dots

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• 作者：Fangmao Ye ; Changfeng Wu ; Yuhui Jin ; Yang-Hsiang Chan ; Xuanjun Zhang ; Daniel T. Chiu
• 刊名：Journal of the American Chemical Society
• 出版年：2011
• 出版时间：June 1, 2011
• 年：2011
• 卷：133
• 期：21
• 页码：8146-8149
• 全文大小：780K
• 年卷期：v.133,no.21(June 1, 2011)
• ISSN：1520-5126

文摘

This communication describes ultrabright single-nanoparticle ratiometric temperature sensors based on semiconducting polymer dots (Pdots). We attached the temperature sensitive dye鈥擱hodamine B (RhB), whose emission intensity decreases with increasing temperature鈥攚ithin the matrix of Pdots. The as-prepared Pdot-RhB nanoparticle showed excellent temperature sensitivity and high brightness because it took advantage of the light harvesting and amplified energy transfer capability of Pdots. More importantly, the Pdot-RhB nanoparticle showed ratiometric temperature sensing under a single wavelength excitation and has a linear temperature sensing range that matches well with the physiologically relevant temperatures. We employed Pdot-RhB for measuring intracellular temperatures in a live-cell imaging mode. The exceptional brightness of Pdot-RhB allows this nanoscale temperature sensor to be used also as a fluorescent probe for cellular imaging.