Conjugation of a Water-Soluble Gadolinium Endohedral Fulleride with an Antibody as a Magnetic Resonance Imaging Contrast Agent

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• 作者：Chun-Ying Shu ; Xin-Yong Ma ; Jian-Fei Zhang ; Frank D. Corwin ; Jae Hyun Sim ; Er-Yun Zhang ; Harry C. Dorn ; Harry W. Gibson ; Panos P. Fatouros ; Chun-Ru Wang ; Xiao-Hong Fang
• 刊名：Bioconjugate Chemistry
• 出版年：2008
• 出版时间：March 2008
• 年：2008
• 卷：19
• 期：3
• 页码：651 - 655
• 全文大小：881K
• 年卷期：v.19,no.3(March 2008)
• ISSN：1520-4812

文摘

Water-soluble gadofullerides exhibited high efficiency as magnetic resonance imaging (MRI) contrast agents. In this paper, we report the conjugation of the newly synthesized gadofulleride, Gd@C₈₂O₆(OH)₁₆(NHCH₂CH₂COOH)₈, with the antibody of green fluorescence protein (anti-GFP), as a model for “tumor targeted” imaging agents based on endohedral metallofullerenes. In this model system, the activity of the anti-GFP conjugate can be conveniently detected by green fluorescence protein (GFP), leading to in vitro experiments more direct and facile than those of tumor antibodies. Objective-type total internal reflection fluorescence microscopy revealed that each gadofulleride aggregate conjugated on average five anti-GFPs, and the activity of anti-GFPs was preserved after conjugation. In addition, the gadofulleride/antibody conjugate exhibited higher water proton relaxivity (12.0 mM⁻¹ s⁻¹) than the parent gadofulleride aggregate (8.1 mM⁻¹ s⁻¹) in phosphate buffered saline at 0.35 T, as also confirmed by T₁-weighted images of phantoms. These observations clearly indicate that the synthesized gadofulleride/antibody conjugate not only has targeting potential, but also exhibits higher efficiency as an MRI contrast agent.