Ordered Mesoporous Silica Matrix for Immobilization of Chloroperoxidase with Enhanced Biocatalytic Performance for Oxidative Decolorization of Azo Dye

详细信息    查看全文

• 作者：Ruijuan Jiao ; Yi Tan ; Yucheng Jiang ; Mancheng Hu ; Shuni Li ; Quanguo Zhai
• 刊名：Industrial & Engineering Chemistry Research
• 出版年：2014
• 出版时间：August 6, 2014
• 年：2014
• 卷：53
• 期：31
• 页码：12201-12208
• 全文大小：443K
• ISSN：1520-5045

文摘

Mesoporous silica material was prepared to immobilize chloroperoxidase (CPO) from Caldariomyces fumago in this work. The mesoporous material (FDU-12-140) with 15鈥?8 nm pore entrances was found to be a very suitable support not only because it allowed the entry of only a few CPO molecules (diameter around 6.2 nm) so as to avoid the aggregation of enzyme molecules that may result in a decline in its apparent activity, but also because it provided enough flip space for the enzyme during its catalytic action. Enzymatic oxidative decolorization of Crocein Orange G was employed to evaluate the catalytic performance of CPO. The decolorization efficiency of dye by immobilized CPO reached 90% within 70 min. Moreover, it displayed improved thermostability and maintained higher activity in organic solvents compared to that of free enzyme. When incubated at 80 掳C for 1 h, 42% of the activity of immobilized enzyme was reserved compared with that at 20 掳C, whereas free CPO maintained only 8.5%. Even in the most hydrophilic N,N-dimethylformamide (log鈥?i>p = 鈭?.0), the decolorization efficiency remained 30% by immobilized CPO, but no decolorization was observed by free CPO at the same conditions. The relative activity of immobilized CPO in the 20th reuse maintained 61% of that in the first run in decolorization of the dye.