Probing Chitosan and Phospholipid Interactions Using Langmuir and Langmuir-Blodgett Films as Cell Membrane Models

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• 作者：Felippe J. Pavinatto ; Luciano Caseli ; Adriana Pavinatto ; David S. dos Santos ; Jr. ; Thatyane M. Nobre ; Maria E.D. Zaniquelli ; Heurison S. Silva ; Paulo B. Miranda ; Osvaldo N. de Oliveira ; Jr.
• 刊名：Langmuir
• 出版年：2007
• 出版时间：July 3, 2007
• 年：2007
• 卷：23
• 期：14
• 页码：7666 - 7671
• 全文大小：269K
• 年卷期：v.23,no.14(July 3, 2007)
• ISSN：1520-5827

文摘

The interaction between chitosan and Langmuir and Langmuir-Blodgett (LB) films of dimyristoyl phosphatidicacid (DMPA) is investigated, with the films serving as simplified cell membrane models. At the air-water interface,chitosan modulates the structural properties of DMPA monolayers, causing expansion and decreasing the monolayerelasticity. As the surface pressure increased, some chitosan molecules remained at the interface, but others wereexpelled. Chitosan could be transferred onto solid supports alongside DMPA using the LB technique, as confirmedby infrared spectroscopy and quartz crystal microbalance measurements. The analysis of sum-frequency vibrationspectroscopy data for the LB films combined with surface potential measurements for the monolayers pointed tochitosan inducing the ordering of the DMPA alkyl chains. Furthermore, the morphology of DMPA LB films, studiedwith atomic force microscopy, was affected significantly by the incorporation of chitosan, with the mixed chitosan-DMPA films displaying considerably higher thickness and roughness, in addition to chitosan aggregates. Becausechitosan affected DMPA films even at pressures characteristic of cell membranes, we believe this study may helpelucidate the role of chitosan in biological systems.