Inkjet Nanoinjection for High-Thoughput Chemiluminescence Immunoassay on Multicapillary Glass Plate

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• 作者：Fengming Chen ; Sifeng Mao ; Hulie Zeng ; Shuhua Xue ; Jianmin Yang ; Hizuru Nakajima ; Jin-Ming Lin ; Katsumi Uchiyama
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：August 6, 2013
• 年：2013
• 卷：85
• 期：15
• 页码：7413-7418
• 全文大小：320K
• 年卷期：v.85,no.15(August 6, 2013)
• ISSN：1520-6882

文摘

We report a novel chemiluminescence diagnosis system for high-throughput human IgA detection by inkjet nanoinjection on a multicapillary glass plate. As proof-of-concept, microhole-based polydimethylsiloxane (PDMS) sheets were aligned on a multicapillary glass plate to form a microwell array as microreactors for enzyme-linked immunosorbent assay (ELISA). The multicapillary glass plate was utilized as a switch that controlled the holding/passing of the solution. Further, anti-IgA-labeled polystyrene (PS) microbeads was assembled into the microwell array, and an inkjet nanoinjection was specially used to distribute the sample and reagent solution for chemiluminescence ELISA, enabling high-throughput detection of human IgA. As a result, the performance of human IgA tests revealed a wider range for the calibration curve and a lower limit of detection (LOD) of 0.1 ng mL^鈥? than the ELISA by a standard 96-well plate. The analysis time and reagent consumption were significantly decreased. The IgA concentrations in saliva samples were determined after 10000-fold dilution by the developed ELISA system showing comparable results by conventional immune assay with 96-wells. Thus, we believe that the inkjet nanoinjection for high-throughput chemiluminescence immunoassay on a multicapillary glass plate will be promising in disease diagnosis.