Synthesis, Characterization and Antibacterial Activity against Gram Positive and Gram Negative Bacteria of Biomimetically Coated Silver Nanoparticles

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• 作者：Elvio Amato ; Yuri A. Diaz-Fernandez ; Angelo Taglietti ; Piersandro Pallavicini ; Luca Pasotti ; Lucia Cucca ; Chiara Milanese ; Pietro Grisoli ; Cesare Dacarro ; Jose M. Fernandez-Hechavarria ; Vittorio Necchi
• 刊名：Langmuir
• 出版年：2011
• 出版时间：August 2, 2011
• 年：2011
• 卷：27
• 期：15
• 页码：9165-9173
• 全文大小：854K
• 年卷期：v.27,no.15(August 2, 2011)
• ISSN：1520-5827

文摘

In the present work, we describe a simple procedure to produce biomimetically coated silver nanoparticles (Ag NPs), based on the postfunctionalization and purification of colloidal silver stabilized by citrate. Two biological capping agents have been used (cysteine Cys and glutathione GSH). The composition of the capped colloids has been ascertained by different techniques and antibacterial tests on GSH-capped Ag NPs have been conducted under physiological conditions, obtaining values of Minimum Inhibitory Concentration (MIC) of 180 and 15 渭g/mL for Staphylococcus aureus and Escherichia coli, respectively. The antibacterial activity of these GSH capped NPs can be ascribed to the direct action of metallic silver NPs, rather than to the bulk release of Ag⁺.