Novel Approach for Large-Scale, Biocompatible, and Low-Cost Fractionation of Peptides in Proteolytic Digest of Food Protein Based on the Amphoteric Nature of Peptides

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• 作者：Asuka Akahoshi ; Kenji Sato ; Yuko Nawa ; Yasushi Nakamura ; and Kozo Ohtsuki
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2000
• 出版时间：May 2000
• 年：2000
• 卷：48
• 期：5
• 页码：1955 - 1959
• 全文大小：101K
• 年卷期：v.48,no.5(May 2000)
• ISSN：1520-5118

文摘

A large-scale, biocompatible, and low-cost procedure for peptide fractionation based on the amphotericnature of peptide is developed. A sample cell (120 × 100 × 50 mm) with four joint tubes (17 mm i.d.and 20 mm in length) on the front and back was prepared. On the end of the joint tubes, a nylonscreen (100 mesh)-supported agarose gel layer was formed. Five or nine of the sample cells wereconnected. A tryptic digest of casein (2.0-3.6 L) was applied to the sample cells. At each end of thesample cell apparatus, an additional cell filled with 0.1 M H₃PO₄ or NaOH was connected and usedas anode and cathode compartments, respectively. Reproducible fractionation of peptide could beachieved by collecting fractions with specific pH values when voltage reached a plateau by applyingdirect current at constant power. Running time necessary for fractionation of peptide was inverselyproportional to electric power and directly proportional to sample volume.Keywords: Peptide fractionation; preparative isoelectric focusing; autofocusing; electrophoresis;functional foods