Modularized CRISPR/dCas9 Effector Toolkit for Target-Specific Gene Regulation
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- 作者：Michael Agne ; Ilona Blank ; Alica J. Emhardt ; Christoph G. G盲belein ; Fenja Gawlas ; Nadine Gillich ; Patrick Gonschorek ; Thomas J. Juretschke ; Stefan D. Kr盲mer ; Natalie Louis ; Anne M眉ller ; Alina Rudorf ; Lisa M. Sch盲fer ; Manuel C. Scheidmann ; Lisa J. Schmunk ; Philipp M. Schwenk ; Maximilian R. Stammnitz ; Philipp M. Warmer ; Wilfried Weber ; Adrian Fischer ; Beate Kaufmann ; Hanna J. Wagner ; Gerald Radziwill
- 刊名：ACS Synthetic Biology
- 出版年：2014
- 出版时间：December 19, 2014
- 年：2014
- 卷：3
- 期：12
- 页码：986-989
- 全文大小：248K
- ISSN：2161-5063
文摘
The ability to control mammalian genes in a synergistic mode using synthetic transcription factors is highly desirable in fields of tissue engineering, stem cell reprogramming and fundamental research. In this study, we developed a standardized toolkit utilizing an engineered CRISPR/Cas9 system that enables customizable gene regulation in mammalian cells. The RNA-guided dCas9 protein was implemented as a programmable transcriptional activator or repressor device, including targeting of endogenous loci. For facile assembly of single or multiple CRISPR RNAs, our toolkit comprises a modular RNAimer plasmid, which encodes the required noncoding RNA components.