Antioxidant Activity and Inhibition of -Glucosidase by trans-Resveratrol, Piceid, and a Novel trans-Stilbene from the
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- 作者：Zohar Kerem ; Itzhak Bilkis ; Moshe A. Flaishman ; and Lior Sivan
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2006
- 出版时间：February 22, 2006
- 年：2006
- 卷：54
- 期：4
- 页码：1243 - 1247
- 全文大小：74K
- 年卷期：v.54,no.4(February 22, 2006)
- ISSN：1520-5118
文摘
The roots of Rumex bucephalophorus, collected in Israel, were analyzed for trans-stilbenes. Twostilbene-O-glycosyl derivatives were identified, in addition to 3,5,4'-trihydroxystilbene (1) (resveratrol).The stilbene-O-glycosyl derivatives were 5,4'-dihydroxystilbene-3-O-
-D-glucopyranoside (2) (piceid)and the new 5,4'-dihydroxystilbene-3-O-
-arabinopyranoside (3), which is being named rumexoid.The structure of rumexoid was elucidated by using spectroscopic data. The antioxidant capacities ofstilbenoids 1-3 were determined and expressed as trolox equivalent antioxidant capacity (TEAC).TEAC value for trans-resveratrol was highest (2.7) and for rumexoid lowest (1.5). In vitro, trans-resveratrol and rumexoid demonstrated a potent inhibitory effect on -glucosidase activity (IC50 <0.1 and < 0.5 mM, respectively). The commercial antidiabetic agent acarbose was shown to inhibitonly 35% of the enzyme activity at 0.5 mM. The addition of piceid to the reaction mixture did notinhibit -glucosidase in vitro in the range of concentrations used. These findings extend the range ofreported beneficial effects of stilbene derivatives, and demonstrate the multifaceted activities thatdietary polyphenols may exert in the intestine, where their concentrations are highest in the body.Keywords: Antioxidants; -glucosidase; trans-resveratrol; piceid; trans-stilbene; Rumex bucephalophorus L.
-D-glucopyranoside (2) (piceid)and the new 5,4'-dihydroxystilbene-3-O--arabinopyranoside (3), which is being named rumexoid.The structure of rumexoid was elucidated by using spectroscopic data. The antioxidant capacities ofstilbenoids 1-3 were determined and expressed as trolox equivalent antioxidant capacity (TEAC).TEAC value for trans-resveratrol was highest (2.7) and for rumexoid lowest (1.5). In vitro, trans-resveratrol and rumexoid demonstrated a potent inhibitory effect on -glucosidase activity (IC50 <0.1 and < 0.5 mM, respectively). The commercial antidiabetic agent acarbose was shown to inhibitonly 35% of the enzyme activity at 0.5 mM. The addition of piceid to the reaction mixture did notinhibit -glucosidase in vitro in the range of concentrations used. These findings extend the range ofreported beneficial effects of stilbene derivatives, and demonstrate the multifaceted activities thatdietary polyphenols may exert in the intestine, where their concentrations are highest in the body.Keywords: Antioxidants; -glucosidase; trans-resveratrol; piceid; trans-stilbene; Rumex bucephalophorus L.