Biomimetic Cryptic Site Surfaces for Reversible Chemo- and Cyto-Mechanoresponsive Substrates
详细信息 查看全文
- 作者：Jalal Bacharouche ; Florent Badique ; Ahmad Fahs ; Maria V. Spanedda ; Alexandre Geissler ; Jean-Pierre Malval ; Marie-France Vallat ; Karine Anselme ; Gr茅gory Francius ; Benoit Frisch ; Joseph Hemmerl茅 ; Pierre Schaaf ; Vincent Roucoules
- 刊名：ACS Nano
- 出版年：2013
- 出版时间：April 23, 2013
- 年：2013
- 卷：7
- 期：4
- 页码：3457-3465
- 全文大小：501K
- 年卷期：v.7,no.4(April 23, 2013)
- ISSN：1936-086X
文摘
Chemo-mechanotransduction, the way by which mechanical forces are transformed into chemical signals, plays a fundamental role in many biological processes. The first step of mechanotransduction often relies on exposure, under stretching, of cryptic sites buried in adhesion proteins. Likewise, here we report the first example of synthetic surfaces allowing for specific and fully reversible adhesion of proteins or cells promoted by mechanical action. Silicone sheets are first plasma treated and then functionalized by grafting sequentially under stretching poly(ethylene glycol) (PEG) chains and biotin or arginine-glycine-aspartic acid (RGD) peptides. At unstretched position, these ligands are not accessible for their receptors. Under a mechanical deformation, the surface becomes specifically interactive to streptavidin, biotin antibodies, or adherent for cells, the interactions both for proteins and cells being fully reversible by stretching/unstretching, revealing a reversible exposure process of the ligands. By varying the degree of stretching, the amount of interacting proteins can be varied continuously.