Oligonucleotide-Based Fluorescence Probe for Sensitive and Selective Detection of Mercury(II) in Aqueous Solution
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- 作者：Cheng-Kang Chiang ; Chih-Ching Huang ; Chi-Wei Liu ; Huan-Tsung Chang
- 刊名：Analytical Chemistry
- 出版年：2008
- 出版时间：May 15, 2008
- 年：2008
- 卷：80
- 期：10
- 页码：3716 - 3721
- 全文大小：186K
- 年卷期：v.80,no.10(May 15, 2008)
- ISSN：1520-6882
文摘
In this paper we unveil a new homogeneous assay
using TOTO-3 and the polythymine oligonucleotide T33for the highly selective and sensitive detection of Hg2+ in aqueous solution. The fluorescence of TOTO-3 is weak in the absence or presence of randomly coiled T33. After T33 interacts specifically with Hg2+ ions through T−Hg2+−T bonding, however, its conformation changes to form a folded structure that preferably binds to TOTO-3. As a result, the fluorescence of a mixture of T33 and TOTO-3 increases in the presence of Hg2+. Our data from fluorescence polarization spectroscopy, capillary electrophoresis with laser-induced fluorescence detection, circular dichroism spectroscopy, and melting temperature measurements confirm the formation of folded T33−Hg2+ complexes. Under optimum conditions, the TOTO-3/T33 probe exhibited a high selectivity (≥265-fold) toward Hg2+ over other metal ions, with a limit of detection of 0.6 ppb. We demonstrate the practicality of this TOTO-3/T33 probe for the rapid determination of Hg2+ levels in pond water and in batteries. This approach offers several advantages, including rapidity (<15 min), simplicity (label-free), and low cost.
using TOTO-3 and the polythymine oligonucleotide T33for the highly selective and sensitive detection of Hg2+ in aqueous solution. The fluorescence of TOTO-3 is weak in the absence or presence of randomly coiled T33. After T33 interacts specifically with Hg2+ ions through T−Hg2+−T bonding, however, its conformation changes to form a folded structure that preferably binds to TOTO-3. As a result, the fluorescence of a mixture of T33 and TOTO-3 increases in the presence of Hg2+. Our data from fluorescence polarization spectroscopy, capillary electrophoresis with laser-induced fluorescence detection, circular dichroism spectroscopy, and melting temperature measurements confirm the formation of folded T33−Hg2+ complexes. Under optimum conditions, the TOTO-3/T33 probe exhibited a high selectivity (≥265-fold) toward Hg2+ over other metal ions, with a limit of detection of 0.6 ppb. We demonstrate the practicality of this TOTO-3/T33 probe for the rapid determination of Hg2+ levels in pond water and in batteries. This approach offers several advantages, including rapidity (<15 min), simplicity (label-free), and low cost.