Non-Invasive Vibrational SFG Spectroscopy Reveals That Bacterial Adhesion Can Alter the Conformation of Grafted 鈥淏rush鈥?Chains on SAM

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• 作者：Emilie Bulard ; Ziang Guo ; Wanquan Zheng ; Henri Dubost ; Marie-Pierre Fontaine-Aupart ; Marie-Noe虉lle Bellon-Fontaine ; Jean-Marie Herry ; Romain Briandet ; Bernard Bourguignon
• 刊名：Langmuir
• 出版年：2011
• 出版时间：April 19, 2011
• 年：2011
• 卷：27
• 期：8
• 页码：4928-4935
• 全文大小：958K
• 年卷期：v.27,no.8(April 19, 2011)
• ISSN：1520-5827

文摘

Understanding bacterial adhesion on a surface is a crucial step to design new materials with improved properties or to control biofilm formation and eradication. Sum Frequency Generation (SFG) vibrational spectroscopy has been employed to study in situ the conformational response of a self-assembled monolayer (SAM) of octadecanethiol (ODT) on a gold film to the adhesion of hydrophilic and hydrophobic ovococcoid model bacteria. The present work highlights vibrational SFG spectroscopy as a powerful and unique non-invasive biophysical technique to probe and control bacteria interaction with ordered surfaces. Indeed, the SFG vibrational spectral changes reveal different ODT SAM conformations in air and upon exposure to aqueous solution or bacterial adhesion. Furthermore, this effect depends on the bacterial cell surface properties. The SFG spectral modeling demonstrates that hydrophobic bacteria flatten the ODT SAM alkyl chain terminal part, whereas the hydrophilic ones raise this ODT SAM terminal part. Microorganism-induced alteration of grafted chains can thus affect the desired interfacial functionality, a result that should be considered for the design of new reactive materials.