One Metabolite, Two Pathways: Convergence of Polypropionate Biosynthesis in Fungi and Marine Molluscs
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- 作者：Adele Cutignano ; Guido Villani ; Angelo Fontana
- 刊名：Organic Letters
- 出版年：2012
- 出版时间：February 17, 2012
- 年：2012
- 卷：14
- 期：4
- 页码：992-995
- 全文大小：224K
- 年卷期：v.14,no.4(February 17, 2012)
- ISSN：1523-7052
文摘
Structural similarity or even the identity of polyketide compounds does not necessarily imply unique biosynthesis. Feeding experiments with a 13C labeled precursor establish that the C3 units in 7-methyl-cyercene-1 (1) are derived from intact propionate in the marine mollusc Ercolania funerea. The same compound in the terrestrial fungus Leptosphaeria maculans/Phoma lingam is synthesized by an acetate/SAM pathway thus proving for the first time metabolic convergence of polyketide biosynthesis in eukaryotes. Traditional 1H鈥?sup>13C NMR correlation spectroscopy has been successfully applied to estimate 13C incorporation in biosynthetic experiments.