The Sodium Ions Inside a Lipophilic G-Quadruplex Channel as Probed by Solid-State 23Na NMR

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• 作者：Alan Wong ; James C. Fettinger ; Scott L. Forman ; Jeffery T. Davis ; and Gang Wu
• 刊名：Journal of the American Chemical Society
• 出版年：2002
• 出版时间：February 6, 2002
• 年：2002
• 卷：124
• 期：5
• 页码：742 - 743
• 全文大小：69K
• 年卷期：v.124,no.5(February 6, 2002)
• ISSN：1520-5126

文摘

We report solid-state ²³Na NMR and X-ray crystallographic results for a self-assembled G-quadruplex channel formed by a guanine nucleoside, 5'-tert-butyl-dimethylsilyl-2',3'-O-isopropylidene guanosine (G 1). The study provides an unambiguous ²³Na NMR identification for the Na⁺ ions inside a lipophilic G-quadruplex channel. The crystalline nature of the sample yields a remarkably high resolution in the ²³Na multiple-quantum magic-angle spinning (MQMAS) spectrum, making it possible to extract very accurate ²³Na NMR parameters for each of the three crystallographically distinct Na sites. The observation of a single Na⁺ ion from a 9-kDa system demonstrates the potential of solid-state ²³Na NMR as a complementary technique to X-ray for detecting Na⁺ ions in biological structures.