Amphiphilic Self-Assemblies Decorated by Nucleobases

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• 作者：Debora Berti ; Francesca Baldelli Bombelli ; Martina Fortini ; Piero Baglioni
• 刊名：Journal of Physical Chemistry B
• 出版年：2007
• 出版时间：October 11, 2007
• 年：2007
• 卷：111
• 期：40
• 页码：11734 - 11744
• 全文大小：1846K
• 年卷期：v.111,no.40(October 11, 2007)
• ISSN：1520-5207

文摘

Phosphatidyl-nucleosides are a class of functional amphiphiles, where a nucleic acid monomer is conjugatedto a lipid skeleton. These derivatives self-organize in aqueous solution as assemblies of various size, shape,and interfacial curvature. This paper presents a comparison of the aggregation behavior of different 1-R,2-R-sn-glycero-3-phosphatidyl-nucleosides, where R = 8 (DiC₈PN) or R = 12 (DLPN) and N is either adenosine(a purine) or uridine (a pyrimidine), a complementary pair in RNA. Surface tension, small angle neutronscattering, cryo-TEM, and circular dichroism are used to highlight and distinguish the impact of the hydrophobicassembler and of the base substitution on the solution phase behavior. Our main conclusion is that the nucleicfunctionalization provides an additional parameter to control self-assembly through specific interactions amongthe polar heads. Further nonideal effects are induced by mixing nucleolipids with complementary basesubstitution. We show that these contributions alter the aggregation thresholds and modulate properties of theaggregates on the mesoscale.