Generation of Roasted Notes Based on 2-Acetyl-2-thiazoline and Its Precursor, 2-(1-Hydroxyethyl)-4,5-dihydrothiazole, by Combined Bio and Thermal Approaches
文摘
Roasted notes contribute to the flavor of thermally processed foods such as meat and bread. 2-Acetyl-2-thiazoline is one of the key volatile compounds responsible for the roasted and popcorn-like aromacharacter. We report here on the biogeneration of flavoring preparations with intense roasted notes,which are characterized by a high content of 2-acetyl-2-thiazoline. These flavoring preparations wereobtained by fermentation of cysteamine, ethyl-L-lactate, and D-glucose with baker's yeast. Theprecursor of 2-acetyl-2-thiazoline, 2-(1-hydroxyethyl)-4,5-dihydrothiazole, was prepared under mildconditions by microbial reduction of the carbonyl group of 2-acetyl-2-thiazoline using baker's yeastas biocatalyst. The addition of 2-(1-hydroxyethyl)-4,5-dihydrothiazole as aroma precursor to pizzadough resulted in an increase of the roasted note.Keywords: Roasted notes; bioflavor; baker's yeast; baked flavor; 2-acetyl-2-thiazoline; 2-(1-hydroxyethyl)-4,5-dihydrothiazole; GC-Olfactometry; GC-MS