Pseudomonas fluorescens N21.4 Metabolites Enhance Secondary Metabolism Isoflavones in Soybean (Glycine max) Calli Cultures
详细信息 查看全文
- 作者：Elena Algar ; Francisco Javier Gutierrez-Ma帽ero ; Alfonso Bonilla ; Jose Antonio Lucas ; Wojtek Radzki ; Beatriz Ramos-Solano
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2012
- 出版时间：November 7, 2012
- 年：2012
- 卷：60
- 期：44
- 页码：11080-11087
- 全文大小：381K
- 年卷期：v.60,no.44(November 7, 2012)
- ISSN：1520-5118
文摘
Phytopharmaceuticals are plant secondary metabolites that are strongly inducible and especially sensitive to biotic changes. Plant cell cultures are a good alternative to obtain secondary metabolites, in case effective stimulation can be achieved. In this study, metabolic elicitors from two rhizobacteria able to enhance isoflavone content in soybean seedlings were tested on three different soybean calli cell lines. Results show that metabolic elicitors from Chryseobacterium balustinum Aur9 were not effective. However, there are at least two different metabolic elicitors from Pseudomonas fluorescens N21.4, one under 10 kDa and another over 10 kDa, that trigger isoflavone metabolism in the three cell lines with different isoflavone content. Elicitors from N21.4 achieved total isoflavone increases up to 29.7% (0.205 mg/g), 64.5% (0.487 mg/g), and 23.4% (0.726 mg/g) in the low-, intermediate-, and high-yield lines, respectively. Therefore, these elicitors have a great potential to enhance isoflavone production in cell cultures for development of functional ingredients.