Hydrogel Scaffolds with Immobilized Bacteria for 3D Cultures

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• 作者：Mar&iacute ; a C. Gutié ; rrez ; Zaira Y. Garc&iacute ; a-Carvajal ; Mat&iacute ; as Jobbá ; gy ; Luis Yuste ; Fernando Rojo ; Concepció ; n Abrusci ; Fernando Catalina ; Francisco del Monte ; Mar&iacute
• 刊名：Chemistry of Materials
• 出版年：2007
• 出版时间：April 17, 2007
• 年：2007
• 卷：19
• 期：8
• 页码：1968 - 1973
• 全文大小：422K
• 年卷期：v.19,no.8(April 17, 2007)
• ISSN：1520-5002

文摘

We have studied the suitability of a cryogenic process (e.g., ice-segregation-induced self-assembly,ISISA) for preparation of polyvinyl alcohol (PVA) scaffolds with in situ immobilized bacteria (e.g.,Escherichia coli). Confocal fluorescence microscopy and impedance measurements were used to evaluatethe extension of bacteria proliferation within the scaffold macrostructure. The bacteria efficiency forcolonization of the scaffold macrostructure is compared for bacteria immobilized with and without theuse of additional cryoprotectans. Cryoprotection by bacteria entrapment in alginate beads containingglucose results in a significant improvement (more than 2-fold as compared to non-cryoprotected) ofbacteria proliferation within the PVA scaffold. Results are also compared with the most widely usedmethod for cells colonization of scaffolds; i.e., soaking of a preformed PVA scaffold in bacteria culturemedium.