Preparation of Calcium Alginate Nanoparticles Using Water-in-Oil (W/O) Nanoemulsions

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• 作者：Alexandra H. E. Machado ; Dan Lundberg ; Ant贸nio J. Ribeiro ; Francisco J. Veiga ; Bj枚rn Lindman ; Maria G. Miguel ; Ulf Olsson
• 刊名：Langmuir
• 出版年：2012
• 出版时间：March 6, 2012
• 年：2012
• 卷：28
• 期：9
• 页码：4131-4141
• 全文大小：466K
• 年卷期：v.28,no.9(March 6, 2012)
• ISSN：1520-5827

文摘

A procedure for the preparation of calcium alginate nanoparticles in the aqueous phase of water-in-oil (W/O) nanoemulsions was developed. The emulsions were produced from mixtures of the nonionic surfactant tetraethylene glycol monododecyl ether (Cb>12b>Eb>4b>), decane, and aqueous solutions of up to 2 wt % sodium alginate by means of the phase inversion temperature (PIT) emulsification method. This method allows the preparation of finely dispersed emulsions without a large input of mechanical energy. With alginate concentrations of 1鈥? wt % in the aqueous phase, emulsions showed good stability against Ostwald ripening and narrow, monomodal distributions of droplets with radii <100 nm. Gelation of the alginate was induced by the addition of aqueous CaClb>2b> to the emulsions under stirring, and particles formed were collected using a simple procedure based on extraction of the surfactant on addition of excess oil. The final particles were characterized using cryo-transmission electron microscopy (cryo-TEM) and dynamic light scattering (DLS). They were found to be essentially spherical with a homogeneous interior, and their size was similar to that of the initial emulsion droplets. The herein presented 鈥渓ow-energy鈥?method for preparation of biocompatible nanoparticles has the potential to be used in various applications, e.g., for the encapsulation of sensitive biomacromolecules.