Thermo-Regulated Adhesion of the Streptococcus thermophilus螖rgg0182 Strain

详细信息    查看全文

• 作者：Gr茅gory Francius ; Romain Henry ; J茅r么me F. L. Duval ; Emmanuelle Bruneau ; Jenny Merlin ; Ahmad Fahs ; Nathalie Leblond-Bourget
• 刊名：Langmuir
• 出版年：2013
• 出版时间：April 16, 2013
• 年：2013
• 卷：29
• 期：15
• 页码：4847-4856
• 全文大小：458K
• 年卷期：v.29,no.15(April 16, 2013)
• ISSN：1520-5827

文摘

The physicochemical determinants governing the temperature-dependent adhesion of Streptococcus thermophilus to abiotic surfaces are identified under physiological condition for cells either lacking or not the Rgg₀₁₈₂ transcriptional regulator involved in their thermal adaptation. For that purpose, the wild type LMG18311 strain and 螖rgg₀₁₈₂ mutant were imaged using highly resolved atomic force microscopy (AFM) at various cell growth temperatures (42 to 55 掳C). The corresponding hydrophobic/hydrophilic balance of the cells was quantitatively addressed via the measurement by chemical force microcopy of their adhesion to a reference hydrophobic surface. Analysis of force鈥搒eparation distance curves further allowed us to discriminate cell surfaces according to the presence or absence of biopolymers. These results were interpreted in relation to the measured adhesion of the 螖rgg₀₁₈₂ mutant onto the hydrophobic wall of microwells in the temperature range from 46 to 52 掳C. It is evidenced that the viscoelastic 螖rgg₀₁₈₂ cell envelop behaves as a thermo-responsive film whose hydrophobicity increases with increasing temperature, thereby favoring cell attachment to hydrophobic surfaces. Regardless cell growth temperature, wild-type cells do not attach to hydrophobic surfaces and the presence of the Rgg₀₁₈₂ transcriptional regulator is associated with the synthesis of hydrophilic cell surface biopolymers. Throughout, the impact of electrostatics on bioadhesion is ruled out upon examination of electrohydrodynamic cell properties at 50 掳C.