Supported Lipid Bilayer Formation and Lipid-Membrane-Mediated Biorecognition Reactions Studied with a New Nanoplasmonic Sensor Template

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• 作者：Magnus P. Jonsson ; Peter Jö ; nsson ; Andreas B. Dahlin ; Fredrik Hö ; ö ; k
• 刊名：Nano Letters
• 出版年：2007
• 出版时间：November 2007
• 年：2007
• 卷：7
• 期：11
• 页码：3462 - 3468
• 全文大小：301K
• 年卷期：v.7,no.11(November 2007)
• ISSN：1530-6992

文摘

This paper presents the use of the localized surface plasmon resonance (LSPR) sensor concept to probe the formation of macroscopic andlaterally mobile supported lipid bilayers (SLBs) on SiO_x-encapsulated nanohole-containing Au and Ag films. A comparison between Au- andAg-based sensor templates demonstrates a higher sensitivity for Au-based templates with respect to both bulk and interfacial refractive index(RI) changes in aqueous solution. The lateral mobility of SLBs formed on the SiO_x-encapsulated nanohole templates was analyzed usingfluorescence recovery after photobleaching (FRAP), demonstrating essentially complete (>96%) recovery, but a reduction in diffusivity ofabout 35% compared with SLBs formed on flat SiO_x substrates. Furthermore, upon SLB formation, the temporal variation in extinction peakposition of the LSPR active templates display a characteristic shape, illustrating what, to the best of our knowledge, is the first example wherethe nanoplasmonic concept is shown capable of probing biomacromolecular structural changes without the introduction of labels. With asignal-to-noise ratio better than 5 × 10² upon protein binding to the cell-membrane mimics, the sensor concept is also proven competitive withstate-of-the-art label-free sensors.