Real-Time Volumetric Phase Monitoring: Advancing Chemical Analysis by Countercurrent Separation

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• 作者：Guido F. Pauli ; Samuel M. Pro ; Lucas R. Chadwick ; Thomas Burdick ; Luke Pro ; Warren Friedl ; Nick Novak ; John Maltby ; Feng Qiu ; J. Brent Friesen
• 刊名：Analytical Chemistry
• 出版年：2015
• 出版时间：July 21, 2015
• 年：2015
• 卷：87
• 期：14
• 页码：7418-7425
• 全文大小：416K
• ISSN：1520-6882

文摘

Countercurrent separation (CCS) utilizes the differential partitioning behavior of analytes between two immiscible liquid phases. We introduce the first platform (鈥淐herryOne鈥? capable of real-time monitoring, metering, and control of the dynamic liquid鈥搇iquid CCS process. Automated phase monitoring and volumetrics are made possible with an array of sensors, including the new permittivity-based phase metering apparatus (PMA). Volumetric data for each liquid phase are converted into a dynamic real-time display of stationary phase retention (Sf) and eluent partition coefficients (K), which represent critical parameters of CCS reproducibility. When coupled with the elution鈥揺xtrusion operational mode (EECCC), automated Sf and K determination empowers untargeted and targeted applications ranging from metabolomic analysis to preparative purifications.