Carbon in Intimate Contact with Quartz Reduces the Biological Activity of Crystalline Silica Dusts

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• 作者：Mara Ghiazza ; Maura Tomatis ; Sophie Doublier ; Francesca Grendene ; Elena Gazzano ; Dario Ghigo ; Bice Fubini
• 刊名：Chemical Research in Toxicology
• 出版年：2013
• 出版时间：January 18, 2013
• 年：2013
• 卷：26
• 期：1
• 页码：46-54
• 全文大小：387K
• 年卷期：v.26,no.1(January 18, 2013)
• ISSN：1520-5010

文摘

To evaluate the effect of carbonaceous materials on the pathogenic activity of quartz dusts, mixtures of carbon soot (1 and 10%) and quartz (Min-U-Sil) were prepared and then milled so to attain an intimate association of carbon and the quartz surface. Both cellular and cell-free tests show that carbon associated to quartz completely inhibits the typical free radical generation of quartz dusts (through Fenton activity and homolytic cleavage of a C鈥揌 bond) and suppresses the oxidative stress and inflammation induced by quartz alone on MH-S murine macrophage cells (lipid peroxidation, nitric oxide release, and tumor necrosis factor-伪 synthesis). The cytotoxic response to quartz is also largely reduced. An extremely pure quartz milled with 10% of soot showed inactivating effects on the adverse reactions to quartz similar to Min-U-Sil quartz. None of these effects takes place when the same experiments are carried out with mechanically mixed samples, which suggests that carbon acts not just as a radical quencher but because of its association to the quartz surface.