Imogolite: An Aluminosilicate Nanotube Endowed with Low Cytotoxicity and Genotoxicity

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• 作者：Bianca Maria Rotoli ; Patrizia Guidi ; Barbara Bonelli ; Margherita Bernardeschi ; Massimiliano G. Bianchi ; Serena Esposito ; Giada Frenzilli ; Paolo Lucchesi ; Marco Nigro ; Vittoria Scarcelli ; Maura Tomatis ; Pier Paolo Zanello ; Bice Fubini ; Ovidio Bussolati ; Enrico Bergamaschi
• 刊名：Chemical Research in Toxicology
• 出版年：2014
• 出版时间：July 21, 2014
• 年：2014
• 卷：27
• 期：7
• 页码：1142-1154
• 全文大小：666K
• ISSN：1520-5010

文摘

High-aspect-ratio nanomaterials (HARN) (typically, single-walled carbon nanotubes (SWCNT) or multiwalled carbon nanotubes (MWCNT)) impair airway barrier function and are toxic to macrophages. Here, we assess the biological effects of nanotubes of imogolite (INT), a hydrated alumino-silicate [(OH)₃Al₂O₃SiOH] occurring as single-walled NT, on murine macrophages and human airway epithelial cells. Cell viability was assessed with resazurin. RT-PCR was used to study the expression of Nos2 and Arg1, markers of classical or alternative macrophage activation, respectively, and nitrite concentration in the medium was determined to assess NO production. Epithelial barrier integrity was evaluated from the trans-epithelial electrical resistance (TEER). Potential genotoxicity of INT was assessed with comet and cytokinesis-block micronucleus cytome assays. Compared to MWCNT and SWCNT, INT caused much smaller effects on RAW264.7 and MH-S macrophage viability. The incubation of macrophages with INT at doses as high as 120 渭g/cm² for 72 h did not alter either Nos2 or Arg1 expression nor did it increase NO production, whereas IL6 was induced in RAW264.7 cells but not in MH-S cells. INT did not show any genotoxic effect in RAW264.7 and A549 cells except for a decrease in DNA integrity observed in epithelial A549 cells after treatment with the highest dose (80 渭g/cm²). No significant change in permeability was recorded in Calu-3 epithelial cell monolayers exposed to INT, whereas comparable doses of both SWCNT and MWCNT lowered TEER. Thus, in spite of their fibrous nature, INT appear not to be markedly toxic for in vitro models of lung鈥揵lood barrier cells.