Intercalation Interactions between dsDNA and Acridine Studied by Single Molecule Force Spectroscopy
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- 作者：Chuanjun Liu ; Zhenhua Jiang ; Yiheng Zhang ; Zhiqiang Wang ; Xi Zhang ; Fude Feng ; Shu Wang
- 刊名：Langmuir
- 出版年：2007
- 出版时间：August 28, 2007
- 年：2007
- 卷：23
- 期：18
- 页码：9140 - 9142
- 全文大小：106K
- 年卷期：v.23,no.18(August 28, 2007)
- ISSN：1520-5827
文摘
In this letter, we report on the direct measurement of the intercalation interactions between acridine and double-stranded DNA (dsDNA) using single molecule force spectroscopy. The interaction between acridine and dsDNA isbroken by force of 36 pN at a loading rate of 5.0 nN/s. The most probable rupture force between acridine and dsDNAis dependent on the loading rate, indicating that the binding of acridine and dsDNA is a dynamic process. Thecombination of SMFS experimental data with the theoretical model clearly suggests the presence of two energy barriersalong with an unbinding trajectory of acridine-dsDNA.