DNA Photo-Cross-Linking Using 3-Cyanovinylcarbazole Modified Oligonucleotide with Threoninol Linker

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• 作者：Takashi Sakamoto ; Yuya Tanaka ; Kenzo Fujimoto
• 刊名：Organic Letters
• 出版年：2015
• 出版时间：February 20, 2015
• 年：2015
• 卷：17
• 期：4
• 页码：936-939
• 全文大小：322K
• ISSN：1523-7052

文摘

3-Cyanovinylcarbazole modified d-threoninol (^CNVD) was incorporated in oligodeoxyribonucleotide and tested for a photo-cross-linking reaction with complementary oligodeoxyribonucleotide. The photoreactivity was 1.8- to 8-fold greater than that of 3-cyanovinylcarbazole modified deoxyribose (^CNVK) previously reported. From the results of melting analysis and circular dichroism spectroscopy of the duplexes, the relatively flexible structure of ^CNVD compared with ^CNVK might be advantageous for [2 + 2] photocycloaddition between the cyanovinyl group on the ^CNVD and pyrimidine base in the complementary strand.