Autoxidized Phospholipids in Hexane: Nano-Self-Assemblies Studied by Synchrotron Small-Angle X-ray Scattering

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• 作者：Eleonora V. Shtykova ; Vladimir V. Volkov ; Hua-Jie Wang ; Tetsuro Fujisawa ; Jin-Ye Wang
• 刊名：Langmuir
• 出版年：2006
• 出版时间：September 12, 2006
• 年：2006
• 卷：22
• 期：19
• 页码：7994 - 8000
• 全文大小：124K
• 年卷期：v.22,no.19(September 12, 2006)
• ISSN：1520-5827

文摘

Synchrotron small-angle X-ray scattering (SAXS) was used to analyze the structure of self-assembled autoxidizedphospholipids in a very dilute solution of hexane. In addition, it was used to build a self-consistent model of theaggregates, taking into account their inner heterogeneities and polydispersity. The scattering intensity from a dilutemixture of different types of noninteracting components of the phospholipid system was represented as a linearcombination of partial intensities from the components weighted by their volume fractions. Applying this approachthe final model of the system was described as a mixture of polydisperse reverse micelles and aggregates with sphericaland cylindrical shapes. Spherical aggregates were represented as hollow spheres with inner radius 0.7 nm (occupiedby water or hexane) and outer radius 1.5 nm. Geometrical parameters of the aggregates did not change much duringthe oxidation process, while the ratio of reverse micelles and aggregates in solution varied. The amount of the reversemicelles increased from very low to about 80%, whereas the content of other aggregates constantly reduced. Theanalysis performed in this study helps one to better understand the processes of phospholipid oxidation, which mayoccur in biological membranes.