NMR-Based Metabolic Profiling of Rice Wines by F2-Selective Total Correlation Spectra

详细信息    查看全文

• 作者：Masanori Koda ; Kazuo Furihata ; Feifei Wei ; Takuya Miyakawa ; Masaru Tanokura
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2012
• 出版时间：May 16, 2012
• 年：2012
• 卷：60
• 期：19
• 页码：4818-4825
• 全文大小：496K
• 年卷期：v.60,no.19(May 16, 2012)
• ISSN：1520-5118

文摘

In this study, we performed NMR-based metabolic profiling of major rice wines (Japanese sake, Chinese Shaoxing wine, and Korean makgeolli). In the ¹H NMR spectra, the rice wines showed broad resonances in the region of about 7.9鈥?.0 ppm. These resonances showed many and complex correlations with approximately 0.5鈥?.5 ppm in the F₂-selective TOCSY (total correlation spectroscopy) spectra, and these correlations were attributed mainly to peptides. These spectral patterns were characteristic of individual rice wines, and the combination of F₂-selective TOCSY spectra and principal component analysis enabled us to classify the rice wine species. Furthermore, it also provided information about raw materials, namely, what type of koji (rice koji or wheat koji) was used. These spectra may be useful as a new 鈥渇ingerprint鈥?for quality control or food authentication.

Keywords:

NMR; TOCSY; selective excitation; metabolic profiling; rice wine; peptide