Development of a Lateral Flow Colloidal Gold Immunoassay Strip for the Rapid Detection of Enrofloxacin Residues
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- 作者：Yinli Zhao ; Gaiping Zhang ; Qingtang Liu ; Man Teng ; Jifei Yang ; Jianhua Wang
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2008
- 出版时间：December 24, 2008
- 年：2008
- 卷：56
- 期：24
- 页码：12138-12142
- 全文大小：194K
- 年卷期：v.56,no.24(December 24, 2008)
- ISSN：1520-5118
文摘
A rapid immunochromatographic lateral flow test strip of competitive format has been developed using a gold-conjugated monoclonal antibody for the specific determination of enrofloxacin (ENR) residues in chicken muscles. For this purpose, a specific monoclonal antibody (mAb) for ENR was generated and characterized. The mAb showed negligible cross-reactivity with other related compounds. Using ENR standards prepared in chicken muscle extracts from 0 to 24.3 ng/mL (μg/kg), the method indicated that the detection limit of the test strip, as measured in a strip scanner, was as low as 0.138 μg/kg of ENR and the half-maximal inhibition concentration (IC50) was 0.935 μg/kg. For samples spiked at 10, 20, and 30 μg/kg, the recovery was between 85.3 and 96.1% and the coefficient of variation [CV (%)] was between 4.5 and 7.91%. Parallel analysis of muscle samples from chickens fed ENR showed good comparable results obtained from the test strip and LC-MS. Each test requires 5−10 min. The data indicate that the method has high sensitivity, specificity, and the advantages of simplicity and speed of performance. Therefore, the test strip provides a useful screening method for quantitative, semiquantitative, or qualitative detection of ENR residues in chicken muscles.