Characterization of Flavonols in Cranberry (Vaccinium macrocarpon) Powder
详细信息 查看全文
- 作者：Irina O. Vvedenskaya ; Robert T. Rosen ; Jane E. Guido ; David J. Russell ; Kent A. Mills ; and Nicholi Vorsa
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2004
- 出版时间：January 28, 2004
- 年：2004
- 卷：52
- 期：2
- 页码：188 - 195
- 全文大小：121K
- 年卷期：v.52,no.2(January 28, 2004)
- ISSN：1520-5118
文摘
Flavonoids were extracted from cranberry powder with acetone and ethyl acetate and subsequentlyfractionated with Sephadex LH-20 column chromatography. The fraction eluted with a 60% methanolsolution was composed primarily of phenolic constituents with maximum absorbance at 340 nm. Ahigh-performance liquid chromatography procedure was developed, which resolved 22 distinct peakswith UV/vis and mass spectra corresponding to flavonol glycoside conjugates. Six new constituentsnot previously reported in cranberry or in cranberry products were determined through NMRspectroscopy to be myricetin-3-
-xylopyranoside, quercetin-3--glucoside, quercetin-3-
-arabinopyranoside, 3'-methoxyquercetin-3--xylopyranoside, quercetin-3-O-(6' '-p-coumaroyl)--galactoside,and quercetin-3-O-(6' '-benzoyl)--galactoside. Quercetin-3-O-(6' '-p-coumaroyl)--galactoside andquercetin-3-O-(6' '-benzoyl)--galactoside represent a new class of cranberry flavonol compoundswith three conjugated components consisting of a flavonol, sugar, and carboxylic acid (benzoic orhydroxycinnamic acids). This is also the first report identifying quercetin-3-arabinoside in both furanoseand pyranose forms in cranberry. Elucidation of specific flavonol glycosides in cranberry is significantsince the specificity of the sugar moiety may play a role in the bioavailability of the flavonol glycosidesin vivo.Keywords: Vaccinium macrocarpon Ait.; cranberry; flavonol glycosides; quercetin; myricetin; arabinopyranoside; acylated quercetin-3-glycosides
-xylopyranoside, quercetin-3--glucoside, quercetin-3--arabinopyranoside, 3'-methoxyquercetin-3--xylopyranoside, quercetin-3-O-(6' '-p-coumaroyl)--galactoside,and quercetin-3-O-(6' '-benzoyl)--galactoside. Quercetin-3-O-(6' '-p-coumaroyl)--galactoside andquercetin-3-O-(6' '-benzoyl)--galactoside represent a new class of cranberry flavonol compoundswith three conjugated components consisting of a flavonol, sugar, and carboxylic acid (benzoic orhydroxycinnamic acids). This is also the first report identifying quercetin-3-arabinoside in both furanoseand pyranose forms in cranberry. Elucidation of specific flavonol glycosides in cranberry is significantsince the specificity of the sugar moiety may play a role in the bioavailability of the flavonol glycosidesin vivo.Keywords: Vaccinium macrocarpon Ait.; cranberry; flavonol glycosides; quercetin; myricetin; arabinopyranoside; acylated quercetin-3-glycosides